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KMID : 0380620210530060815
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 6 p.815 ~ p.818
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Development of an improved selective media for differentiation of emetic and diarrheal type Bacillus cereus
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Hong Yong-Gun
Lee Jin-Joo
Kim Sang-Soon
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Abstract
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The objective of this study was to develop a differential medium with improved selectivity for the isolation of Bacillus cereus. Mannitol egg yolk polymyxin medium supplemented with D-galactose allowed the differentiation of diarrheal- and emetic-type B. cereus through pH monitoring. The pH of the medium decreased significantly when incubating the emetic-type B. cereus, whereas the pH change was not significant when incubating the diarrheal-type. The addition of pH indicators, such as methyl red and phenol red, to the medium allowed visual differentiation between diarrheal- and emetic-type B. cereus. A solid agar medium was also developed by optimizing the concentrations of medium components such as monosaccharides, agar, egg yolk enrichment, pH indicators, and antibiotics. This study indicates the possibility of applying selective media for the differentiation of diarrheal- and emetic-type B. cereus.
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KEYWORD
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selective media, Bacillus cereus, biochemical property, detection, differentiation
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